I am not convinced that your patient has mast cell activation syndrome but would consider the possibility of idiopathic anaphylaxis. The general criteria for mast cell activation syndrome would include an increase in the baseline serum/blood tryptase to more than 11.5 ng/ml but less than 20 ng/ml or an increase of at least 30% of the basal tryptase during an episode. I do not think the histologic description of the skin biopsy would change my opinion as “slight increase in mast cells” is too nonspecific.
I would not recommend a bone marrow examination or c-KIT mutation studies. I agree with your treatment with high dose antihistamines and would consider adding a leuktotriene modifier. I would also provide epinephrine autoinjectors with instruction for use in flares associated with change in mental status or concern about hypotension. If the severity of the episodes increase, I would repeat the tryptase, try to get a tryptase during an episode and would consider alternative therapies utilized for idiopathic anaphylaxis, including oral corticosteroids. Omalizumab may improve idiopathic anaphylaxis but is off label.
I have included a couple of references you may find of value and a prior Ask The Expert question with additional references.
I have shared my opinion with Dr. Mariana Castells, Professor of Medicine Harvard University and Brigham and Women’s Hospital. Dr. Castells has responded.
“Mast cell activation syndrome MCAS is defined by compatible mast cell activation symptoms in at least 2 organ systems, the presence of elevated mast cell mediators at baseline or during acute episodes and the response to mast cell controller medications. The patient has clear symptoms of mast cell activation in 2 organs and he should have metyl histamine and prostaglandin metabolites PG11F2a in 24 hour urine and a KIT mutation for D816V in peripheral blood. Because 10-15% of patients with systemic mastocytosis have tryptase below 20 ng/ml systemic mastocytosis needs to be ruled out. If c-KIT mutation is positive a Bone Marrow Biopsy is indicated. Because of the continued presence of urticaria omalizumab may be indicated. In addition Ketotifen and sodium cromolyn are indicated for MCAS.
Here is the reference for MCAS. I hope this helps your management of this difficult patients
Hamilton MJ, Hornick JL, Akin C, Castells MC, Greenberger NJ. Mast cell activation syndrome: A newly recognized disorder with systemic clinical manifestations. J Allergy Clin Immunol.128(1):147-52 e2. Epub 2011/05/31.”
In summary, I would treat as idiopathic anaphylaxis but with the elevated tryptase and Dr. Castell’s opinion, particularly the issue of 10-15% of patients with mastocytosis with tryptase less than 20 ng/ml, you may want to obtain urinary studies for histamine and Pg11F2a and c-KIT mutation on peripheral blood. If any of these are abnormal, then bone marrow would be suggested by Dr. Castells. My position is that if you are addressing the issue of anaphylaxis and the CBC is normal, I am not sure what you are going to do differently after the bone marrow. Follow up would be different though if your risk concerns are greater. The practice of medicine and allergy/immunology is an art based on science and there are many areas of grey art and not black and white science.
Akin C1, Valent Diagnostic Criteria and Classification of Mastocytosis in 2014. Immunol Allergy Clin North Am. 2014 May;34(2):207-218. doi: 10.1016/j.iac.2014.02.003.
Lee MJ, Akin C. Mast cell activation syndromes. Ann Allergy Asthma Immunol. 2013 Jul;111(1):5-8. doi: 10.1016/j.anai.2013.02.008. Review
Jones, Jeremy D., Samuel R. Marney, and John M. Fahrenholz. "Idiopathic anaphylaxis successfully treated with omalizumab." Annals of Allergy, Asthma & Immunology 101.5 (2008): 550-551.
Anaphylaxis without apparent cause
64 yowf with solitary episode of hypotensive anaphylaxis. PMH: HTN, DM, melanoma. Meds: lisinopril, atenolol (both dc'd since), HCTZ, wellbutrin. Awoke well. Usual breakfast, same foods eaten regularly since. Lunch-beans, rice, sausage; eaten since w/o event. 1 hr later- diarrhea, abd pain. 4 hr later- pruritus, hives, lip swelling then SOB w/o wheezing, stridor, etc. Took Benadryl. In ER, hypotension, IM epi x3; needed Epi drip and glucagon. Stabilized next day then discharged following day. Abd CT showed "fluid in colon", given flagyl.
No other meds, alternative/herbal therapies, NSAIDs, latex, bee/fire ant/tick bites.
No symptoms of mastocytosis, carcinoid, pheo.
No diarrhea/travel/well water.
Normal CBC,diff,metabolic profile, tryptase, urine N methyl histamine, 5HIAA, Latex IgE, gal-alpha-gal IgE, food skin test panel,VIP. Total IgE 274. Urinary Metanephrines 8 (90-315), Norepinephrine 25 (122-676).
Aside from possibly a bone marrow biopsy to further evaluate mastocytosis, other diagnostic considerations/tests
A: I congratulate you on a very thorough evaluation of a very serious event. I cannot suggest any other diagnostic tests that would be appropriate at this time. I would be most concerned about idiopathic anaphylaxis (first episode so difficult to make this diagnosis) or alpha-gal/mammalian meat anaphylaxis despite the negative in vitro test. Dr. Cummins’ group utilize intradermal meat testing to assess this problem in addition to the in vitro test. Gelatin sensitivity has also been reported with mammalian meat sensitive individuals.
I would suggest the following. If another event occurs, I would repeat the tryptase. Even baseline levels less than 11.5 ng/ml may have mast cell activation syndrome and in these cases the tryptase during symptoms is 1.35 X greater than the baseline or 1.2 X baseline + 2 ng/ml (Clin Exp Allergy 2011;41:1777-83). I am not sure when the meat testing and alpha-gal testing were performed in relationship to the event, but I would consider repeat testing if testing was in close proximity (less than 4-6weeks). Although you indicate your patient has eaten the sausage dish, it is possible that the reaction is dose dependent and you may want to have him intentionally ingest a larger amount of the sausage.
Finally, you may consider testing for gelatin allergy with a commercial test available or you can perform skin testing with 1 tsp of Jell-O in 5 ml of normal saline (Kelso J et al. Adverse reactions to vaccines practice parameter 2012 update. J Allergy Clin Immunol 2012;130:25-43). I would not favor a bone marrow with the information provided.
Commins, Scott P., et al. "Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-a-1, 3-galactose." Journal of Allergy and Clinical Immunology 123.2 (2009): 426-433.
Morisset, M., et al. "Anaphylaxis to pork kidney is related to IgE antibodies specific for galactose-alpha-1, 3-galactose." Allergy 67.5 (2012): 699-704.
Mullins, Raymond James, et al. "Relationship between red meat allergy and sensitization to gelatin and galactose-a-1, 3-galactose." Journal of Allergy and Clinical Immunology 129.5 (2012): 1334-1342.
I hope this information is of some help to you and your patient.
All my best.
Dennis K. Ledford, MD, FAAAAI